Tag

Part:BBa_K1992003:Design

Designed by: Shilo Ohayon   Group: iGEM16_Technion_Israel   (2016-10-08)


Tar chemoreceptor tagged with GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1282
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2324
    Illegal SapI.rc site found at 111


Design Notes

The reverse transcription PCR primers used:
GFP:
Forward: '5 - gaagcggcagcggtagcatgcgtaaaggagaagaac -'3
Reverse: '5 - ttatttgtatagttcatccatgc -'3

Tar:
Forward: '5 - atgattaaccgtatccgcgtagtcac -'3
Reverse: '5 - ctcctttacgcatgctaccgctgccgcttccaaatgtttcccagtttgg -'3